3RNT

CRYSTAL STRUCTURE OF GUANOSINE-FREE RIBONUCLEASE T1, COMPLEXED WITH VANADATE(V), SUGGESTS CONFORMATIONAL CHANGE UPON SUBSTRATE BINDING

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	48.820, 46.530, 41.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.137
RMSD bond length	0.025
RMSD bond angle	0.061
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800 *	1.800 *
Total number of observations	11536 *
Number of reflections	7134 *
Completeness [%]	77.0 *	59 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNaseT1	1-2 (%(w/v))
2	1	reservoir	sodium acetate	10 (mM)
3	1	reservoir		2 (mM)
4	1	reservoir		1 (mM)
5	1	drop	MPD	55 (%(v/v))