3RNR
Crystal Structure of Stage II Sporulation E Family Protein from Thermanaerovibrio acidaminovorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97903 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.530, 87.349, 93.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.021 - 2.000 |
| R-factor | 0.1708 |
| Rwork | 0.169 |
| R-free | 0.20220 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.282 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | |
| Number of reflections | 37141 | |
| <I/σ(I)> | 10 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.8 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES (NaOH) pH 7.5, 10 % (w/v) PEG8000, 10 % (v/v) ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
