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3RN5

Structural basis of cytosolic DNA recognition by innate immune receptors

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 23-ID-B
Synchrotron siteAPS
Beamline23-ID-B
Temperature [K]298
Detector technologyCCD
Collection date2010-07-16
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1.0332
Spacegroup nameP 1 21 1
Unit cell lengths62.360, 136.360, 77.040
Unit cell angles90.00, 89.98, 90.00
Refinement procedure
Resolution46.015 - 2.500
R-factor0.2021
Rwork0.201
R-free0.24110
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3rlo
RMSD bond length0.008
RMSD bond angle0.965
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: dev_736))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.590
High resolution limit [Å]2.5002.500
Rmerge0.0530.621
Number of reflections44323
<I/σ(I)>19.263.29
Completeness [%]99.6100
Redundancy4.424.45
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.52988% PEG1000, 0.1 M KCl, 10 mM MgCl2, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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