3RN5
Structural basis of cytosolic DNA recognition by innate immune receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.360, 136.360, 77.040 |
| Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
| Resolution | 46.015 - 2.500 |
| R-factor | 0.2021 |
| Rwork | 0.201 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rlo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_736)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.053 | 0.621 |
| Number of reflections | 44323 | |
| <I/σ(I)> | 19.26 | 3.29 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.42 | 4.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8% PEG1000, 0.1 M KCl, 10 mM MgCl2, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
