3RN4
Crystal structure of iron-substituted Sod2 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 92.690, 92.690, 92.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.950 - 1.790 |
| R-factor | 0.19829 |
| Rwork | 0.196 |
| R-free | 0.23791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bfr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.227 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.950 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 25311 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 45% MPD, 0.1M Bicine pH 9.5, 0.2M NH4H2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
