3RMR

Crystal structure of Hyaloperonospora arabidopsidis ATR1 effector domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0722, 1.0631, 1.11587
Spacegroup name	P 61 2 2
Unit cell lengths	119.432, 119.432, 312.572
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.349 - 2.300
R-factor	0.225
Rwork	0.223
R-free	0.25980
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.022
Data reduction software	ELVES
Data scaling software	ELVES
Phasing software	PHENIX (AutoSol)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.431	2.510
High resolution limit [Å]	2.268	2.420
Rmerge	0.083	0.729
Number of reflections	57939
<I/σ(I)>	35.55	4.375
Completeness [%]	99.4	97.6
Redundancy	15.5	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Crystal growth in 1.6 M magnesium sulfate, 0.1 M MES, pH 6.5. 1:20000 seeding in 1.2 M magnesium sulfate, 0.01% acetonitrile, 0.1 M MES, pH 5.0. Dehydration in 1.5 M magnesium sulfate, 0.1 M MES, pH 5.0 for 2 hours, VAPOR DIFFUSION, HANGING DROP, temperature 291K