3RMP
Structural basis for the recognition of attP substrates by P4-like integrases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.900, 69.490, 83.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.210 |
| R-factor | 0.23625 |
| Rwork | 0.234 |
| R-free | 0.28021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jtz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.847 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.800 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.066 |
| Number of reflections | 14688 |
| <I/σ(I)> | 17.09 |
| Completeness [%] | 81.5 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 65% MPD, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
