3RMH
Crystal Structure of yeast telomere protein Cdc13 OB4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-12-17 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.087, 39.046, 63.100 |
Unit cell angles | 90.00, 108.84, 90.00 |
Refinement procedure
Resolution | 29.859 - 1.902 |
R-factor | 0.2076 |
Rwork | 0.206 |
R-free | 0.23820 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.075 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 22461 |
Completeness [%] | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | sitting drop | 7.4 | 277 | 28%PEG4000, 0.1M Tris-HCl, 0.01M NH4Cl, 0.05M CaCl2, pH 7.4, sitting drop, temperature 277K |