3RLK

Crystal structure of the read-through domain from bacteriophage Qbeta A1 protein, monoclinic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976180
Spacegroup name	P 1 21 1
Unit cell lengths	44.010, 49.120, 44.260
Unit cell angles	90.00, 118.41, 90.00

Refinement procedure

Resolution	38.930 - 1.760
R-factor	0.1768
Rwork	0.174
R-free	0.24180
Structure solution method	MIRAS
RMSD bond length	0.023
RMSD bond angle	1.925
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.930	38.930	1.860
High resolution limit [Å]	1.760	5.570	1.760
Rmerge		0.046	0.334
Total number of observations		1874	7776
Number of reflections	16414
<I/σ(I)>	10	13.4	2.2
Completeness [%]	99.2	99.7	99.5
Redundancy	3.4	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1 M Tris-HCl, 40% PEG 300, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K