3RLF
Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to MgAMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 |
Unit cell lengths | 72.098, 95.812, 109.983 |
Unit cell angles | 86.70, 82.68, 76.40 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.22481 |
Rwork | 0.223 |
R-free | 0.25406 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r6g |
RMSD bond length | 0.007 |
RMSD bond angle | 1.012 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.054 | |
Number of reflections | 127961 | |
<I/σ(I)> | 1.6 | 2 |
Completeness [%] | 88.1 | 56.7 |
Redundancy | 2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 28% PEG 4000, 0.1M sodium hepes pH 7.5, 0.2M sodium chloride, 0.05M magnesium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |