3RLE
Crystal Structure of GRASP55 GRASP domain (residues 7-208)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 88.040, 88.040, 61.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.250 - 1.649 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.22214 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.250 | 50.000 | 1.680 |
| High resolution limit [Å] | 1.649 | 4.480 | 1.649 |
| Number of reflections | 26966 | ||
| <I/σ(I)> | 58 | 2.4 | |
| Completeness [%] | 96.0 | 99.3 | 75.3 |
| Redundancy | 12.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1M imidazole, 0.2M Ammonium Acetate, 25% PEG 400, 10% PEG 20000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
