3RLC

Crystal structure of the read-through domain from bacteriophage Qbeta A1 protein, hexagonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-17
Detector	MAR CCD 165 mm
Wavelength(s)	1.03874
Spacegroup name	P 63 2 2
Unit cell lengths	69.110, 69.110, 167.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.930 - 2.900
R-factor	0.2166
Rwork	0.213
R-free	0.29690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.368
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.555	34.555	3.060
High resolution limit [Å]	2.900	9.170	2.900
Rmerge		0.029	0.543
Total number of observations		1726	7941
Number of reflections	5773
<I/σ(I)>	17.5	18.8	1.4
Completeness [%]	100.0	98.6	100
Redundancy	9.5	7.3	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	40% PEG 300, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K