3RKY
Structural characterisation of staphylococcus aureus biotin protein ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.99510 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 94.266, 94.266, 130.885 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.797 - 3.232 |
R-factor | 0.1942 |
Rwork | 0.192 |
R-free | 0.23920 |
Starting model (for MR) | 3rir |
RMSD bond length | 0.015 |
RMSD bond angle | 1.868 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 9929 |
Completeness [%] | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |