3RKX

Structural characterisation of staphylococcus aureus biotin protein ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.95364
Spacegroup name	P 1 21 1
Unit cell lengths	50.132, 51.401, 67.584
Unit cell angles	90.00, 108.00, 90.00

Refinement procedure

Resolution	34.956 - 2.100
R-factor	0.1965
Rwork	0.193
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rir
RMSD bond length	0.007
RMSD bond angle	1.062
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.100
Number of reflections	18537
Completeness [%]	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Peg 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K