3RKV

C-terminal domain of protein C56C10.10, a putative peptidylprolyl isomerase, from Caenorhabditis elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	42.025, 84.674, 110.731
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.300 - 2.410
R-factor	0.2401
Rwork	0.237
R-free	0.30050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fbn
RMSD bond length	0.019
RMSD bond angle	1.646
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.400	50.000	2.460
High resolution limit [Å]	2.410	6.560	2.420
Rmerge	0.087	0.050	0.815
Number of reflections	7946
<I/σ(I)>	9.2		1.98
Completeness [%]	100.0	100	100
Redundancy	9.3	8.4	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	297	40% MPD, 0.1 M CHES , pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K