3RKS

Crystal Structure of the Manihot esculenta Hydroxynitrile Lyase (MeHNL) K176P mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Detector technology	IMAGE PLATE
Collection date	2009-04-28
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	86.628, 91.414, 137.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.190 - 2.500
R-factor	0.17724
Rwork	0.174
R-free	0.23819
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.500
Number of reflections	36063
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2% PEG 400, 2.0M ammonium sulphate, 0.1M Hepes-Na (pH 7.5), 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K