3RKC
Hepatitis E Virus Capsid Protein E2s Domain (genotype IV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-17 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.714, 66.533, 49.721 |
| Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.790 |
| Rwork | 0.155 |
| R-free | 0.19190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ggq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.736 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 26223 |
| Completeness [%] | 100.0 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1M Tris, 20% PEG 10000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
