3RK6
Crystal structure of the middle domain of human Paip1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-19 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.610, 76.050, 62.420 |
| Unit cell angles | 90.00, 96.47, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.000 |
| R-factor | 0.1922 |
| Rwork | 0.190 |
| R-free | 0.24370 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 | 0.023 |
| Number of reflections | 36673 | |
| <I/σ(I)> | 14.9 | 5.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 18% PEG20000, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
