3RJX
Crystal Structure of Hyperthermophilic Endo-Beta-1,4-glucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.712, 85.210, 53.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.310 - 2.400 |
| R-factor | 0.17639 |
| Rwork | 0.172 |
| R-free | 0.25852 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.670 |
| Number of reflections | 12870 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 1.2M NaH2PO4/KH2PO4 (1:1), 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 1.2M NaH2PO4/KH2PO4 (1:1), 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 1.2M NaH2PO4/KH2PO4 (1:1), 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
