3RJV
Crystal structure of a putative sel1 repeat protein (kpn_04481) from Klebsiella pneumoniae subsp. pneumoniae at 1.65 a resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-23 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.96109,0.97932,0.97915 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.508, 81.508, 83.343 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.136 - 1.650 |
R-factor | 0.1717 |
Rwork | 0.171 |
R-free | 0.19360 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.436 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.136 | 29.136 | 1.690 |
High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
Rmerge | 0.042 | 0.782 | |
Total number of observations | 2214 | 17523 | |
Number of reflections | 38945 | ||
<I/σ(I)> | 10.9 | 16.3 | 1 |
Completeness [%] | 99.9 | 92.9 | 100 |
Redundancy | 6.1 | 4.8 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10.00% Glycerol, 1.2600M tri-Sodium Citrate, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |