3RJS
Crystal structure of Dynein Light Chain 8a (DLC8) from Toxoplasma gondii at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-23 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 75.025, 75.025, 85.081 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.756 - 1.500 |
| R-factor | 0.1938 |
| Rwork | 0.193 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cmi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1060) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.620 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.035 | 0.252 |
| Number of reflections | 14931 | |
| <I/σ(I)> | 46 | 6.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 295 | 16% polyethylene glycol 4000, 0.2 M (NH4)2SO4, 0.1 M CH3COONa, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
