3RJR
Crystal Structure of pro-TGF beta 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934, 0.97932, 0.97956, 0.95667 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.690, 126.940, 137.930 |
| Unit cell angles | 90.00, 96.72, 90.00 |
Refinement procedure
| Resolution | 46.554 - 3.050 |
| R-factor | 0.2748 |
| Rwork | 0.274 |
| R-free | 0.31060 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.488 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (dev_276) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.050 |
| Rmerge | 0.047 |
| Number of reflections | 35401 |
| Completeness [%] | 97.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 7% PEG 3350, 17% isopropanol, 0.1m Na citrate pH 5.6, vapor diffusion, temperature 293K, VAPOR DIFFUSION |
