3RJP
Crystal structure of the DNA binding domain of CovR from Streptococcus pyogenes
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979547, 1.019859, 0.979663 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.467, 36.686, 38.937 |
Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
Resolution | 19.590 - 1.500 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.21900 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.660 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 13184 | |
Completeness [%] | 94.8 | 94.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 14% PEG3350, 50mM Zn(OAc)2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |