3RJ6
Crystal Structure of Horse heart ferric myoglobin; K45E/K63E/K96E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-02-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07809 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.341, 123.565, 34.986 |
Unit cell angles | 90.00, 89.73, 90.00 |
Refinement procedure
Resolution | 61.780 - 1.230 |
R-factor | 0.17425 |
Rwork | 0.173 |
R-free | 0.19737 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.029 |
RMSD bond angle | 2.340 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.780 | 1.261 |
High resolution limit [Å] | 1.230 | 1.230 |
Number of reflections | 64506 | |
Completeness [%] | 97.6 | 92.85 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 3.3 M ammonium sulfate, 0.02 M tris, 0.001 M EDTA, , pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |