3RJ2
Structural and functional characterization of a novel histone H3 binding protein ORF158L from the Singapore grouper iridovirus (SGIV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2007-10-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 60.066, 41.002, 52.386 |
Unit cell angles | 90.00, 95.33, 90.00 |
Refinement procedure
Resolution | 29.900 - 2.200 |
R-factor | 0.20101 |
Rwork | 0.197 |
R-free | 0.27503 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.386 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.000 | |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 6586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 3% glycerol, 18% PEG3350, 0.1M Tris, 0.3M NaAc, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |