3RIZ

Crystal structure of the plant steroid receptor BRI1 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-10
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	175.093, 67.251, 119.050
Unit cell angles	90.00, 121.55, 90.00

Refinement procedure

Resolution	30.989 - 2.523
R-factor	0.1897
Rwork	0.187
R-free	0.23960
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.018
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.680
High resolution limit [Å]	2.520	2.520
Number of reflections	39686
<I/σ(I)>	17.6	2.1
Completeness [%]	98.8	95.3
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	14% PEG4000, 0.2 M ammonium sulfate, 0.1 M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K