3RIU
Crystal structure of Drosophila hexameric C3PO formed by truncated Translin and Trax
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 196.030, 196.030, 155.189 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.881 - 3.400 |
R-factor | 0.2739 |
Rwork | 0.271 |
R-free | 0.33810 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 0.864 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.214 | 0.865 |
Number of reflections | 24690 | |
<I/σ(I)> | 11.4 | 2.7 |
Completeness [%] | 99.8 | |
Redundancy | 14.4 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.35 M-0.7 M (NH4)2HPO4, 0.1 M Na-citrate pH 5.4-5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |