3RIQ
Siphovirus 9NA tailspike receptor binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-23 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 149.132, 149.132, 149.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.350 - 1.500 |
| R-factor | 0.12984 |
| Rwork | 0.128 |
| R-free | 0.16950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.430 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.300 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.071 | 0.755 |
| Number of reflections | 87485 | |
| <I/σ(I)> | 15.6 | 2.59 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.6 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 20% PEG 5000 MME, 0.1M BisTris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
