3RIP

Crystal Structure of human gamma-tubulin complex protein 4 (GCP4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.033
Spacegroup name	P 63 2 2
Unit cell lengths	214.950, 214.950, 128.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.22837
Rwork	0.227
R-free	0.25992
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THE STARTING MODEL WAS SOLVED AND PARTIALLY REFINED FROM SAD DATA COLLECTED USING THE SELENEMETHIONINE PROTEIN
RMSD bond length	0.025
RMSD bond angle	2.105
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	73248
<I/σ(I)>		2.45
Completeness [%]	99.4	99.9
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	285	8-10% MPD (2-methyl-2,4-pentanediol), 0-20% glycerol (w/v), 300-400 mM Tris-Hcl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K