3RID
X-ray structure of the C-terminal swapped dimer of P114A variant of Ribonuclease A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-27 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.481, 96.948, 83.079 |
Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.180 |
R-factor | 0.188 |
Rwork | 0.181 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1F0V |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.260 |
High resolution limit [Å] | 2.180 | 2.180 |
Rmerge | 0.074 | 0.313 |
Number of reflections | 38571 | |
<I/σ(I)> | 14.8 | 4.3 |
Completeness [%] | 99.6 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 17-19% PEG 20000, 0.1M cacodylate, 100-150 mg/ml trehalose, 11mM dCpdG, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |