3RID
X-ray structure of the C-terminal swapped dimer of P114A variant of Ribonuclease A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-27 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.481, 96.948, 83.079 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.180 |
| R-factor | 0.188 |
| Rwork | 0.181 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1F0V |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.074 | 0.313 |
| Number of reflections | 38571 | |
| <I/σ(I)> | 14.8 | 4.3 |
| Completeness [%] | 99.6 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 17-19% PEG 20000, 0.1M cacodylate, 100-150 mg/ml trehalose, 11mM dCpdG, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
