3RHT
Crystal structure of type 1 glutamine amidotransferase (GATase1)-like protein from Planctomyces limnophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-05 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.287, 126.827, 101.964 |
Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.830 |
R-factor | 0.15803 |
Rwork | 0.158 |
R-free | 0.19172 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.440 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.093 | 0.607 |
Number of reflections | 93329 | |
<I/σ(I)> | 19.5 | 2.4 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 3.8 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.16 M calcium acetate, 0.08 M sodium cacodylate, 14.4% PEG8000, 20% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |