3RHI
DNA-binding protein HU from Bacillus anthracis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.984, 43.105, 85.141 |
Unit cell angles | 90.00, 125.65, 90.00 |
Refinement procedure
Resolution | 42.300 - 2.480 |
R-factor | 0.22502 |
Rwork | 0.222 |
R-free | 0.29229 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o97 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.615 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.300 | 50.000 | 2.500 |
High resolution limit [Å] | 2.460 | 6.670 | 2.460 |
Rmerge | 0.079 | 0.044 | 0.630 |
Number of reflections | 13039 | ||
<I/σ(I)> | 8.8 | 1.96 | |
Completeness [%] | 99.8 | 98.9 | 99.4 |
Redundancy | 3.5 | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.1 M ammonium tartrate dibasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |