3RHI
DNA-binding protein HU from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.984, 43.105, 85.141 |
| Unit cell angles | 90.00, 125.65, 90.00 |
Refinement procedure
| Resolution | 42.300 - 2.480 |
| R-factor | 0.22502 |
| Rwork | 0.222 |
| R-free | 0.29229 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o97 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.615 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.300 | 50.000 | 2.500 |
| High resolution limit [Å] | 2.460 | 6.670 | 2.460 |
| Rmerge | 0.079 | 0.044 | 0.630 |
| Number of reflections | 13039 | ||
| <I/σ(I)> | 8.8 | 1.96 | |
| Completeness [%] | 99.8 | 98.9 | 99.4 |
| Redundancy | 3.5 | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.1 M ammonium tartrate dibasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
