3RHC
Crystal structure of the holo form of glutaredoxin C5 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8266 |
| Spacegroup name | H 3 |
| Unit cell lengths | 111.747, 111.747, 58.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.990 - 2.400 |
| R-factor | 0.19126 |
| Rwork | 0.189 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.190 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.990 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.061 |
| Number of reflections | 10043 |
| Completeness [%] | 99.0 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 277 | 0.1 M HEPES, 70 % MPD, pH 7.5, Microbatch, temperature 277K |
