3RHB
Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.527, 39.020, 37.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.250 - 1.200 |
| R-factor | 0.15029 |
| Rwork | 0.150 |
| R-free | 0.17585 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fz9 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.209 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.250 |
| High resolution limit [Å] | 1.200 |
| Rmerge | 0.052 |
| Number of reflections | 26715 |
| <I/σ(I)> | 21.9 |
| Completeness [%] | 94.0 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 277 | 1.6 M ammonium sulfate, 500 mM lithium chloride, Microbatch, temperature 277K |
