3RH8
Crystal Structure of the Light-state Dimer of Fungal Blue-Light Photoreceptor Vivid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.647, 77.473, 54.696 |
Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.750 |
Rwork | 0.226 |
R-free | 0.28740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2PD7 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.098 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
Rmerge | 0.157 | 0.080 | 0.500 |
Number of reflections | 8222 | ||
<I/σ(I)> | 4.9 | ||
Completeness [%] | 98.6 | 99.1 | 97.9 |
Redundancy | 3.9 | 4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 290 | 11-15% PEG 6000, 0.1M HEPES (pH = 7.5 - 8.5), 5% 2-Methyl-2,4-pentanediol, VAPOR DIFFUSION, temperature 290K |