3RGS
Structural and kinetic analysis of the beef liver catalase with the ammonia as a ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.480, 140.720, 229.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.380 - 1.990 |
R-factor | 0.161 |
Rwork | 0.161 |
R-free | 0.19800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4blc |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS (1.3) |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.380 | 2.030 |
High resolution limit [Å] | 1.987 | 1.990 |
Rmerge | 0.094 | 0.303 |
Number of reflections | 172258 | |
<I/σ(I)> | 9.8 | 2.5 |
Completeness [%] | 100.0 | 91.5 |
Redundancy | 5.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 45-60mM Mg Formate mixed with 12-13 mg/ml protein containing 1-5 wt% of NH4OH, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |