3RGL
The crystal structure of glycyl-tRNA synthetase subunit alpha from Campylobacter jejuni subsp. jejuni NCTC in complex with ATP and glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 |
| Unit cell lengths | 112.740, 112.740, 157.753 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.510 - 2.450 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rf1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.155 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.500 |
| High resolution limit [Å] | 2.448 | 2.450 |
| Rmerge | 0.091 | 0.627 |
| Number of reflections | 27496 | |
| <I/σ(I)> | 22.5 | 1.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 289 | 0.1M BIS-TRIS PROPANE:NAOH, 0.7M MAGNESIUM FORMATE, 10% Glycine, 10% ATP, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
