3RGH

Structure of filamin A immunoglobulin-like repeat 10 from Homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2010-08-18
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	36.453, 50.549, 107.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.140 - 2.440
R-factor	0.2339
Rwork	0.229
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	iTASSER homology model
RMSD bond length	0.010
RMSD bond angle	1.177
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.510	2.530
High resolution limit [Å]	2.440	2.440
Rmerge	0.070	0.173
Number of reflections	7677
<I/σ(I)>	13.9	5.5
Completeness [%]	98.4	90.7
Redundancy	5.55	4.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.2 M ammonium acetate, 25%(w/v) PEG 3350, 0.1 M Bis-tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K