3RG0
Structural and functional relationships between the lectin and arm domains of calreticulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9779 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.062, 71.701, 108.386 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.230 - 2.570 |
R-factor | 0.23663 |
Rwork | 0.234 |
R-free | 0.28558 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o0v |
RMSD bond length | 0.006 |
RMSD bond angle | 1.061 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 10321 | |
Completeness [%] | 96.9 | 77.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 25% PEG 1500, 0.1 M MIB buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |