3RFR

Crystal Structure of particulate methane monooxygenase (pMMO) from Methylocystis sp. strain M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	110
Detector technology	CCD
Collection date	2009-11-24
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	107.718, 178.310, 183.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.790 - 2.680
R-factor	0.25094
Rwork	0.249
R-free	0.28129
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.735
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.790	2.753
High resolution limit [Å]	2.680	2.684
Number of reflections	83726
Completeness [%]	88.9	54.44
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M MES, 18% PEG750 MME, 0.03 M glycyl-glycyl-glycine, 0.01 M zinc sulfate , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K