3RFJ
Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.567, 60.836, 87.597 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.040 - 1.780 |
R-factor | 0.22366 |
Rwork | 0.222 |
R-free | 0.26038 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 2.279 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.040 | 1.860 |
High resolution limit [Å] | 1.780 | 1.800 |
Number of reflections | 27691 | |
Completeness [%] | 91.2 | 88.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 25.5% polyethylene glycol 4000, 15%(w/v) glycerol, 0.17M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, temperature 293K |