3RFH
Crystal structure of the yeast RACK1 dimer in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-02-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.010, 165.650, 92.150 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 47.227 - 2.900 |
| R-factor | 0.2169 |
| Rwork | 0.215 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rfg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.972 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.128 | 0.515 |
| Number of reflections | 26256 | |
| <I/σ(I)> | 9.09 | 2.88 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20% polyacrylic acid 5100, 0.05M KCl, 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
