3RFG
Crystal structure of the yeast RACK1 dimer in space group P63
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-05-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9918 |
| Spacegroup name | P 63 |
| Unit cell lengths | 177.440, 177.440, 67.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.081 - 3.900 |
| R-factor | 0.2209 |
| Rwork | 0.219 |
| R-free | 0.25580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dm0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.186 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.093 | 0.381 |
| Number of reflections | 11333 | |
| <I/σ(I)> | 12.03 | 3.29 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 4.1 | 3.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100mM HEPES, 1.4M Ammonium sulfate , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
