3REU

Crystal structure of the archaeal asparagine synthetase A complexed with L-Aspartic acid and Adenosine triphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Detector technology	CCD
Collection date	2006-06-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	58.470, 61.180, 156.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.249 - 1.900
R-factor	0.172
Rwork	0.170
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nnh
RMSD bond length	0.007
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	44981
Completeness [%]	99.3	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	100mM TRis pH7, 0.2M NaCl, 32% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K