3RER
Crystal structure of E. coli Hfq in complex with AU6A RNA and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.970, 52.380, 55.520 |
| Unit cell angles | 106.17, 101.42, 98.95 |
Refinement procedure
| Resolution | 32.890 - 1.700 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.468 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.570 | 27.529 | 1.790 |
| High resolution limit [Å] | 1.699 | 5.370 | 1.700 |
| Rmerge | 0.046 | 0.034 | 0.138 |
| Rmeas | 0.040 | 0.160 | |
| Rpim | 0.020 | 0.080 | |
| Total number of observations | 4824 | 23520 | |
| Number of reflections | 41454 | ||
| <I/σ(I)> | 18.1 | 31.6 | 7.4 |
| Completeness [%] | 95.8 | 91.5 | 94.5 |
| Redundancy | 4 | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 283 | 100mM Cacodylate, 100mM NaCl, 12% PEG 8000, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
