3REJ

2.55 Angstrom Crystal Structure of the Nucleosome Core Particle Assembled with a 146 bp Alpha-Satellite DNA (NCP146b)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.07
Spacegroup name	P 21 21 21
Unit cell lengths	106.067, 109.374, 176.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.850 - 2.550
R-factor	0.2343
Rwork	0.234
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kx4
RMSD bond length	0.009
RMSD bond angle	1.432
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	93.000
High resolution limit [Å]	2.550
Rmerge	0.079
Number of reflections	60540
<I/σ(I)>	17.5
Completeness [%]	91.6
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K