3RED
3.0 A structure of the Prunus mume hydroxynitrile lyase isozyme-1
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Detector technology | CCD |
| Collection date | 2009-02-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 112.019, 113.302, 169.488 |
| Unit cell angles | 89.92, 79.13, 80.83 |
Refinement procedure
| Resolution | 10.970 - 3.030 |
| R-factor | 0.1942 |
| Rwork | 0.191 |
| R-free | 0.25285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gdp |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 143272 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 2.0M Ammonium Sulfate, 0.1M BIS-TRIS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
