3RDS
Crystal structure of the refolded R7-2 streptavidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 57.469, 57.469, 173.492 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.130 - 1.500 |
R-factor | 0.1908 |
Rwork | 0.190 |
R-free | 0.21420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rdm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.543 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.054 | 0.033 | 0.318 |
Number of reflections | 22001 | ||
<I/σ(I)> | 14.8 | ||
Completeness [%] | 92.1 | 87.7 | 88.8 |
Redundancy | 7.1 | 7.1 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 25% PEG 1500, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |