3RDK
Protein crystal structure of xylanase A1 of Paenibacillus sp. JDR-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2009-02-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 165.113, 165.113, 66.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 117.040 - 1.490 |
R-factor | 0.1472 |
Rwork | 0.145 |
R-free | 0.18415 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.029 |
RMSD bond angle | 2.321 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 117.040 |
High resolution limit [Å] | 1.490 |
Rmerge | 0.047 |
Number of reflections | 148538 |
<I/σ(I)> | 33.7 |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 294.5 | Protein to mother liquor at 2:1 ratio. mother liquor, 18% PEG 3350, 100mM HEPES, 200mM MgCl2, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 294.5K |