3RD2
NIP45 SUMO-like Domain 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.13 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.521, 32.446, 32.925 |
| Unit cell angles | 90.00, 96.65, 90.00 |
Refinement procedure
| Resolution | 32.703 - 1.600 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3goe |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.113 |
| Number of reflections | 8880 |
| <I/σ(I)> | 40.6 |
| Completeness [%] | 97.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 273 | 1.7 M ammonium 118 sulfate, 100 mM Bicine pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
