3RCZ
Rad60 SLD2 Ubc9 Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 6 |
Unit cell lengths | 115.232, 115.232, 34.842 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.265 - 1.900 |
R-factor | 0.2023 |
Rwork | 0.198 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3GOE.pdb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.161 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.049 |
Number of reflections | 20990 |
<I/σ(I)> | 30 |
Completeness [%] | 99.1 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 20% PEG 8K, 200 mM imidazole malate buffer, pH 5.8, 600 mM LiNO3 and 1 mM DTT, VAPOR DIFFUSION, SITTING DROP, temperature 293K |